Imed Ben Amor
The acceleration and the quality of post surgery healing remain a major issue in medical practice. The platelet rich plasma (PRP) has been widely investigated and used in many chirurgical feeld like orthopedics, dermatology, odontology and gynecology due to its properties and simplicity. Despite the increasing number of studies, there is no consensus regarding the classification used for different types of PRP. Other studies have shown that mesenchymal stromal cells (MSC) have been found in human pererefiric blood. The quantity and quality of MSCs found in the PRP, seem to directly influence the healing results. We hypothesize that current PRP protocols are deleterious for MSCs so that it would be more effective to decrease the speed and duration of centrifugation. Thus the objective of our study was to determine the best centrifugation speed and time to optimize the composition of PRP, particularly MSC, platelet and leukocyte concentration. We have shown that peripheral blood could be considered as a source of MSCs for regenerative medicine. We develop the concept of “soft centrifugation” to obtain a higher concentration of MSCs in the PRP from a peripheral blood sample. Our study shows that injectable platelet rich fibrine with mesenchymal stromal cells (IPRF with MSC) is easy and cheap to produce with minimal effort and can be prepared at the point of care.
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